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This indicates that during P2, K-TCs have a more favorable environment and thus could maintain stronger intensities than during P1, as analyzed in "TC intensity" section.
Notwithstanding, considering the intensities commonly applied, this duration (i.e. ≤ 2 min) may be somewhat "tight" for certain subjects, especially if associated with very high intensities, which may result in premature fatigue, as stated in the "Intensity" section.
Four items in the pain intensity section of SF-BPI are scored from 0 (no pain) to 10 (worst pain possible), and the seven items in the functional interference section are scored from 0 (no interference) to 10 (complete interference).
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To reduce the background staining intensity, sections were incubated with Tris-buffered saline (TBS, pH 7.6) containing 3% (wt/vol).
To improve signal intensity, sections were incubated in 1 M sodium thiosulphate (pH 7.0) for 10 min at 75°C.
According to staining intensity, sections were graded as follows: 0 (no staining), 1 (weak but detectable staining), 2 (distinct staining), or 3 (intense staining).
To ensure unbiased sampling of images for measurements of fluorescence intensity, sections were coded and microscopic fields in the 3rd and 5th cortical layers were randomly selected in the blue channel in which only cell nuclei and cytoplasm were visible, and large pyramidal neurons were identified by their morphology.
For quantification of 3-NT labeling intensity, sections from matched WT and transgenic animals were identically labeled and photographed, and two regions were marked around each MN: one immediately outlining the circumfrence of the MN and another 10 μm further out.
It has been shown that such damage to the surface-attached biomacromolecules depends strongly on the time of their exposure to negative potentials and can be avoided at highly negative I s tr intensities (sections 5.3 and 7.5), inducing very high rates of potential changes and thus very short exposure times.
Subsequent measurements of the intensity profile (Section 3.2) and the polarization dependence of the response (Section 3.3) were conducted within the linear region of the cell response, i.e. at the photon fluxes producing amplitudes which do not exceed 1/3 of the saturating amplitude A0.
Forces are converted to uplift tensile stresses through analysis of exfoliation sheet geometry (see Methods—"Fracture stress intensity analysis" section).
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