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To partially overcome this limitation we read each sample in replicates, and calculated the average intensity, ignoring zero intensity readings.
Five such independent quantities, three strains and two velocities, have to be simultaneously detected and processed in an appropriate way in order to get intensity readings.
From these intensity readings, the density or attenuation value of the tissue at each point in the slice can be calculated.
Lenses with low magnification typically have low numerical aperture, and consequently the effect of the point spread function and the liquid meniscus effect of small liquid volumes on standard microplates conspire to affect the intensity readings.
The resulting data includes 3D scans of the plants (corn and sorghum at present), along with temperature, humidity and light intensity readings taken at different heights — each of which tend to vary greatly, due to an increasing tendency to plant crops closer together.
An experimentally-derived linear relationship between CD54 protein concentration and αCD54-COIN Raman intensity readings (r2 = 0.8 1) was used to determine that the COINs passed iQC.
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Since light intensity and temperature readings have temporal pseudo-replication, GLMM were fitted, considering the months as continuous random factors, and site and nine plants as categorical random factors.
Therefore, an arbitrary gene g from an array consisting of a total of G genes with K technical replicates each will have summary data μ g, k, σ g, k, M g, k where g = 1,..., G and k = 1,..., K. Log-transformation is first applied on the mean bead intensities for all readings.
Autoradiograms from ISH and receptor binding studies were analyzed using the optical density readings (grey intensity).
What they shared, in Mr. Sherman's readings, was an intensity that rendered their differences in language beside the point.
A slavish devotee of a fictional Italian playwright and madman named Gambini, he sinks into his task with an alarming intensity, trying out various line readings.
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