In this work, the integrated intensity quotients of every dumbbell (R) was calculated as a ratio between the integrated intensity in the group V columns (IAs − Bi) in the whole image and the mean integrated intensity in the group V columns in the GaAs layer (IAs), as R = (I(As − Bi))/IAs.
The cancellation of absorption and extinction errors which occurs on taking intensity quotients using reverse-beam measurements of Friedel pair intensities cannot in general be achieved with modern area-detector instruments (but see footnote 2, §2.2).
This was reconfirmed by observing consistently positive intensity correlation quotient (ICQ) values in the different neurons analyzed (Figure 5B).
The neuronal cell body was set as the region of interest and then the intensity correlation quotient (ICQ) was quantified using ImageJ as described by the McMaster Biophotonics Facility.
The intensity correlation quotient (ICQ) reflects the ratio of the number of positive (Ai a)(Bi b) values to the total number of pixels in the ROI, corrected to a −0.5 (independent staining) to +0.5 (dependent staining) range by subtracting 0.5.
Estradiol, lordosis intensity and quotient statistics were a two-way analysis of variance (ANOVA) followed by Bonferroni post-hoc tests.
The whole cytoplasmic area (except the nucleus) per transfected cell was selected and intensity correlation quotient (ICQ) was calculated using the intensity correlation analysis plug-in for ImageJ to evaluate DISC1/mitochondria staining.
Intensity correlation quotients (ICQ) were calculated for each individual ROI.
In this paper we will describe methods based on intensity differences and quotients that enable x to be refined along with all other parameters.
Litter size, placental scar, embryo survival, estradiol measurements, lordosis quotient, and intensity differences were assessed using two-way analysis of variance (ANOVA) followed by Bonferroni post-hoc tests.
Lordosis quotient and intensity has been moved to Figure 3 figure supplement 1C and D, and now includes totals of all animals, rather than separated by mating.
