The average number of molecules of the target protein n p was derived as: n p = f g n r. n r is the known number of molecules of the reference, f is the median of the fluorescence intensity of the patches of the target protein and g is the median fluorescence intensity of the patches of the reference protein.
Each frame was excited with X-Cite 120Q lamp for 400 ms. Frames were not processed for background subtraction and the spots were quantified by quantifying the fluorescence intensity of the patches, in the frame of the z-stack in which they were brighter, and subtracting their local background, as described in Joglekar et al. (2006).
Furthermore, the likelihood of having a negative patch test result at the second test was significantly higher if the intensity of the positive patch test result in the first study was low than if it was high (++ or +++).
In order to estimate the maximum current amplitude, we applied graded mechanical stimuli of increasing intensity until the patch clamp seal became unstable.
The intensities of the fluorescent patches of the target endocytic proteins where in general dimmer than Nuf2, and their distribution was in general not symmetric.
A tree T in a forest F = {T i } is built from the set of annotated patches P = {P i = (I i,c i )} randomly extracted from the training images, where I i and c i are the intensity of patches and the annotated head pose class labels, respectively.
The weights are based on the vector distances of the patch intensities between various non-local patches and the reference patch.
The ePD images of ESC colonies showed that the number and intensity of red patches were lowest under the LIF condition (Fig. 4b, +LIF), intermediate under the LIF condition (Fig. 4b, −LIF), and highest under RA condition (Fig. 4b, +RA).
To decrease the sensitivity of intensity, the census transform [21, 22, 25], which relies on the relative ordering of intensities, successfully characterizes the patch structure and achieves good matching property for accurate disparity estimation near depth discontinuities.
The intensities measured from the patches of the target proteins were thus processed after a logarithmic transformation.
Moreover, it seems to convey information on different time scales since its general development reflects genetic and/or condition at the time of formation and growth, whilst the colour intensity of its yellow patch signals current health status (Locatello et al. 2012).
