Chen et al. [ 76] found that when transcriptional regulatory sequences, transcription factors, and target genes were the same, expression level of luciferase and luminous intensity of substrates could truly reflect the expression of the target genes.
Incision activity was determined, from the average of triplicate lanes (on separate gels), as the intensity of product bands relative to the combined intensities of substrate and product bands.
Due to the fact that SERS hot spots are confined to a small area but dominate the overall SERS intensity of the substrate, the distribution of SERS intensity in a SERS substrate was found to be inhomogeneous, thus limiting their application as reproducible and ultrasensitive sensing platforms.
The studies of Almaas et al., [37], [38] have shown that the internal metabolic activities are adjusted to environmental changes through two distinct mechanisms: Flux plasticity, involving changes in the active catalytic reactions when the organism is shifted from one growth condition to another; these reactive reorganizations result in changes in the intensity of the substrate fluxes.
The intensity of GusB substrate fluorescence was stronger in OA fibroblasts as compared with those isolated from patients with RA.
The fluorescence intensity of the substrate was read in kinetics mode for 30 min at excitation and emission wavelengths of 365 and 445 nm, respectively.
The percentage of HDR was calculated using the following equation (b + c / a + b + c) × 100, where 'a' is the band intensity of DNA substrate and 'b' and 'c' are the cleavage products.
The TE frequency was measured using a T7 endonuclease I assay and calculated using the following equation (1 − (1 − (b + c / a + b + c))1/2 ) × 100, where 'a' is the band intensity of DNA substrate and 'b' and 'c' are the cleavage products (Ran et al., 2013).
The average fluorescence intensity of a substrate-only control droplet was subtracted from the average fluorescence intensity of a MMP substrate combination droplet (with the same substrate concentration), and this intensity difference was converted to a substrate concentration difference using the standard curve in section 4 of the Supporting Information.
The spectra are normalized to the intensity of Si-substrate peak at 520 cm-1.
