Sentence examples for intensity of spots from inspiring English sources

To determine if ncRNA candidates showed detectable expression as assessed by microarray analysis, we used log2 SNR (signal to noise ratios), the "noise" value being the average intensity of spots containing spotting buffer.

The average intensity of spots was determined by scanning using FluorChem software.

Subtraction of fluorescence levels after total destaining (see Materials and Methods) resulted in a difference-image, in which the intensity of spots corresponded to the stimulation-dependent loss of fluorescence and thereby marked active synaptic terminals (see Figure 1A).

To analyze the intensity of spots and to correct for background phosphorylation, the ScanAnalyze software and grid tools were used, and the resulting data were exported to an excel sheet.

One reason could be the relatively low intensity of spots in the CBB gels.

Protein concentration in 2D gels is assessed by measuring the average intensity of spots.

Importantly, SAP-dependent differences in relative signal intensity were reproducibly detected in inverse replicate labeled experiments, in which the intensity of spot 1 24 was reduced upon SAP treatment, and the intensity of spot 1 22 increased after SAP treatment.

Mean intensity of spot and background segments was derived, and expression ratios were calculated as the ratio between the background-subtracted spot intensities of the red (R) and green (G) channel (R/G).

The intensities of spots were analyzed by using PDQuest software ver.8.0 (BioRad Lab).

The locations and intensities of spots in gels from whole cell lysates differed considerably.

Relative intensities of spots in the gels were estimated by PDQuest analysis.