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However, increasing the fluorescence intensity of reference cell population beyond what we were able to achieve would be beneficial.
Each gene signal intensity of microarrays B to H was standardized by the mean intensity of reference microarray A (unfractionated RNA).
However, the relative intensity of reference bands may not be independent from alterations of the molecular structure (Barsberg 2010; Barsberg et al. 2011), and, thus, making use of the entire spectral information (multivariate calibration) is beneficial for regression models (Ferraz et al. 2000).
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The intensities of reference compounds were normalized by their intensities detected on day 1.
As shown in Figure 3, the intensities of reference spots (circled) were essentially unchanged in the various gels, confirming that the differences in amounts of GLP3 isoforms are not due to differences in sample load.
The first normalization step accounted for variations in the mass spectrometer performance, separately for the training and the validation sets, by equating median intensities of reference transitions between the runs.
To evaluate the impact of FCCP treatments on the level of TMRM fluorescence in individual samples, the fluorescence intensities of reference cell populations stained for 45 min with TMRM were compared to fluorescence levels in cell populations which underwent a subsequent FCCP challenge for 20 min.
Besides, a simplified formula based on a point signal is proposed, which shows a clear physical insight that the shifting selectivity is the square of Fourier transform of the intensity distribution of reference pattern and does not depend on thickness of the disc when a point signal is applied.
GeneXreference was intensity value of reference RNA.
In unpaired analysis, this reference intensity was taken as the average allele intensity of all reference (45 normal lung samples, see above) samples that were heterozygous for a given SNP.
The dynamic light scattering and static light scattering at 25 °C indicated that 11 out of 15 SCP tags merely affected the hydrodynamic radius and light scattering intensity of our reference variants BPTI-19A and BPTI-C2G.
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