Scale bar 10 μm Fig. 7 The fluorescence intensities of fusion protein-coexpressing strains.
In RNA from engineered 293 cells, the signal intensity of positive fusion oligonucleotide (for EWS/FLI 7/6 or EWS/ERG 7/8 fusion) was much higher than most negative fusion oligonucleotides.
The data were normalized by dividing signal intensity of each fusion oligonucleotide for EWS/FLI 7/6 by the average signal intensities of all other fusion oligonucleotides.
We see the intensity of this fusion of originality and individuality whenever a plagiarism scandal erupts.
The intensity of nuclear fusion reactions in a plasma is derived by averaging the product of the particles' speed and their cross sections over a distribution of speeds corresponding to a Maxwell-Boltzmann distribution.
The intensity of the fusion protein fluorescent signal in the nucleus and cytoplasm was compared quantitatively with acquisition settings described above.
The purity and intensity of all fusion proteins was determined by Coomassie Brilliant Blue R (Sigma) staining.
The fluorescence intensity of each fusion protein is also substantially greater than that of the corresponding untagged target protein, even when that protein contains multiple tryptophan residues [ 12, 28, 29].
Comparable results were found with SAS-6::GFP (Supplementary information, Figure S1G-1I), altheugh the centriolar GFP signal became undetectable earlier in this case, possibly due to the overall dimmer intensity of this fusion protein compared to GFP::SAS-4.
In the initiating young cell plates, disc-like structures were observed, starting from diffuse expression probably during accumulation of vesicles and increased intensity during fusion of vesicles.
On the other hand, in the WGEP expression system, the fluorescence intensities of HaloTag-fusion proteins from five fusion constructs in the pTD2-Gw vector were slightly reduced compared with those from pTD2-Flx fusion constructs (Fig. 2B, lower lanes: 3, 4, 8 10).
