Sentence examples for intensity of fret from inspiring English sources

Thus, the calculation of FRETc depends on the following relationship: FRETc = IFRET CCFP CYFP, where IFRET corresponds to the intensity of FRET with the FRET filter set of CFP/YFP-coexpressing cells; CCFP and CYFP correspond respectively to the crosstalk between CFP and YFP due to overlapping spectra.

Intensity of the FRET fluorophores was corrected as follows: <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0007877.e001.PNG" class= inline-graphic"/> <img src="http://journals.plos.org/plosone/article/asset?id=info:doi/10.1371/journal.pone.0007877.e002.PNG" class="inline-graphic"/> FRET ratios were then calculated as R = inline-graphic

The tractions are used to determine the effects of fretting fatigue by use of the Stress Intensity Factor.

FRET was finally quantified by calculating the FRET ratio: 1where FD,pre and FD,post are the pre- and post-bleaching intensities of the FRET donor after donor excitation, respectively.

MCAM uses the change of the stress intensity factor (ΔK-parameter) as a fretting fatigue crack initiation parameter, since the change is consistent with the cyclic mechanism of fretting fatigue.

A day or so of fretting followed.

Not free of fretting about Seth.

The acceptor photo-bleaching method was used to determine the intensities of interactions (FRET efficiency) of the C-terminal truncated αA-crystallins with αAwt and αBwt.

The photoluminescence intensity of the patterned FRET sensor increases linearly with increasing concentration of glucose from 0.03 mmol/L to 3 mmol/L, which covers the range of tear glucose levels for both diabetics and healthy subjects.

The fluorescence of the FRET species, IFRET, is a function of the total intensity, ITOT, and the fractional fluorescence resulting from the FRET species, fFRET, according to eq 1, 2 A similar relationship pertains to the non-FRET species, INON-FRET 3 Applying eqs 1– 3 to each pixel enables fluorescence images of FRET and non-FRET states to be produced.

Forskolin and IBMX separately also elicited similar changes of FRET intensity, consistent with the interpretation that changes of intracellular [cAMP] generate in the emission ratio (F470/F535, lower trace Figure 4A) [3].