Blocking of the CD44 receptor decreased fluorescence intensity of cells, and GSH addition increased fluorescence intensity of cells again.
Fluorescence intensity of cells was increased again when GSH was added, indicating that HAssLG nanoparticles have CD44 receptor targetability and potential of redox-responsive drug delivery.
To semiquantitatively assess affinity of the optical probes to the CCK2R, we employed an empirical method, which is based on the determination of the fluorescence intensity of cells after probe incubation according to [26].
In the flow cytometry data (Fig. 6), the histograms of fluorescence intensity of cells that were incubated with various concentrations of FITC-CS@MNPs for 2 h were displayed, and data showed that the number of labeled cells and the mean value of fluorescence intensity followed the incubation concentration of FITC-CS@MNPs.
The mean fluorescence intensity of cells was quantitatively analyzed.
In particular, the ALP staining intensity of cells in the PRG scaffold is significant.
We determined the fluorescence intensity values corresponding to: FA, the fluorescence intensity of cells incubated at 37°C; FB, the fluorescence intensity of cells incubated at 4°C; FC, the fluorescence intensity of glycine- treated cells.
Fluorescence intensity of cells was initially recorded in the basal condition followed by stimulation with 18 mM glucose.
We measured the Raman signal intensity of cells using the IRBA and a 532 nm excitation laser.
To deal with this problem, we examined the individual intensity of cells transiently transfected with GFP-MSH6 and created threshold ranges that grouped cells with similar intensity and produced the correct localization pattern observed for endogenous wild type MSH6.
There is no correlation between fluorescence intensity of cells and their fluorescence lifetime as can be inferred from the 2D histogram (figure 2d) in which the intensity is plotted against the lifetime on a pixel-by-pixel basis.
