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It indicates a decreasing intensity of cell proliferation that led to a reduction of alive cell number at low concentrations of SWCNTs at 60%to70%0% in comparison with the control (Figure 3B).
The explanation may be that the faster growth rate and the stronger intensity of cell metabolism in exponential phase led to the declined strain vitality, shortening target product synthesis phase.
The mRFP intensity of each cell was measured using imageJ software (NIH, Bethesda, MD) and the mean gray values were regarded as the intensity of cell.
The significant differences between autism and controls included both differences in cell counts and, separately, differences in the intensity of cell surface marker expression.
As indicated in Table 4, there were many significant differences in the intensity of cell surface markers expressed on immune cells.
For example, at the p<0.05 level there were 151 variables where either cell count or the intensity of cell surface markers were different between children with autism and controls (Table 2).
Similar(22)
Blocking of the CD44 receptor decreased fluorescence intensity of cells, and GSH addition increased fluorescence intensity of cells again.
Fluorescence intensity of cells was increased again when GSH was added, indicating that HAssLG nanoparticles have CD44 receptor targetability and potential of redox-responsive drug delivery.
After two washes (PBS-albumin, 300 g, 10 min, 25°C), and fixation (2% paraformaldehyde in PBS, 30 min) the levels of phagocytosis were revealed by the florescent intensity of cell-engulfed beads, and could be identified using fluorescence flow cytometry (FACScalibur, BD Biosciences).
The mean fluorescence intensity of cells was quantitatively analyzed.
In particular, the ALP staining intensity of cells in the PRG scaffold is significant.
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