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The intensity of blots was quantified with densitometry using Image Lab software (Bio-Rad).
The intensity of blots was quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).
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The intensity of blot signals was quantitated using ImageQuant TL analysis software (General Electric, UK).
Band intensities of blots against CREB, pCREB, pRSK1/2 and pMSK1 were corrected with the intensities of the loading control actin and set equal to 1 at t = 0.
Quantity One 4.1.1 program (Bio-Rad) was used to quantify the band intensities of blots.
Then, the gray intensities of blots were measured using ImageJ software (Bethesda, MD, USA) and were normalized for β-actin or B23.
The blots were visualized using Immobilon Western (Millipore), and the intensity of the blots was analyzed using a Gel-Pro Analyzer version 4.0 (Media Cybernetics, Bethesda, MD, USA).
The intensity of the blots was measured using Image J (version 1.32; National Institutes of Health, USA) software.
Quantitative assessments of the relative intensity of the blots were analyzed using Image J.
Signal intensity of Western blots was quantitated using a PhosphoImager (Kodak, Rochester, NY, USA).
In rest experiments, the intensity of Western blot signals was quantitated by using an Odyssey Infrared Imaging System (LI-COR Bioscience, Lincoln, Nebraska).
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