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The data show a distinct shift on the intensity axis following the increased deposition temperature.
If the vibration velocity is proportional to the particle velocity (a linear system), the data points of the magnitude should be parallel to the intensity axis.
To better compare subsets of probes in a normalized manner and to allow for statistical evaluation we converted the intensity axis into a rank-axis.
The intensity axis is scaled to photons detected using the detector response calibration provided by the manufacturer.
Therefore, membrane permeabilization is determined by a horizontal population shift that occurs down the green fluorescent intensity axis.
In the interest of clarity, the spectra have been equally spaced with respect to the intensity axis utilising the 1450 peak as a reference point.
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In the intensity-axis method (corresponding to the black vertical lines in Fig. 2), the reflectance spectra must be transformed to the molar extinction coefficient, which has a linear relationship with the concentration, in order to determine the oxygenated levels of hemoglobin; this is because the raw reflectance data is nonlinear with respect to the optical path length.
Y-axis, fluorescence intensity; X-axis, time (min).
(F ) Quantification of P6 GluR2 immunofluorescence intensity (left axis) and P15 GluR2 puncta number per IHC (right axis) in control and Mafb CE cochleae.
Y-axis, relative fluorescence intensity; X-axis, time in minutes (min).
where is the point of intersection of circular curve and logarithm curve, is empirically calibrated to be 15% of the mapped intensity (vertical axis), and and are the origin and radius of the mapping circle.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com