The mean fluorescence intensity (MFI) and coefficient of variation (CV) were also obtained by flow cytometry.
Flow cytometric analysis of the HL60/p53-RNAi HL60/p53-RNAi line displayed dsGFP fluoreporter with a mean fluorescelle intensity of 1721 and coefficient of variation (CV) of 36.8.
Then, we compared four metabolite extraction methods Based on the number of metabolites extracted, their intensity and their coefficient of variation (%CV), the most reproducible protocol (one-step extraction with acetonitrile on lyophilized material) was chosen to further evaluate the impact of sample mass on method performance (3, 6, and 9 mg were essayed).
Based on variance between biological replications after normalization and exclusion of any genes flagged "non-present" for one of the biological replicates, the mean, standard deviation, and coefficient of variation of signal intensity over biological replicates was 7.70, 0.20, and 3.18%, respectively.
The hybrid crack element (HCE) is one of the most accurate and convenient finite elements (FEs) for the direct calculation of the stress intensity factor (SIF) and coefficients of the higher order terms of the Williams expansion.
The THz PSF is mathematically modeled through incorporating the spectrum of the THz imaging system, the axis of propagation of the beam, and the intensity extinction coefficient of the object into a Gaussian beam distribution.
We identified PFCs by the retention time and the intensity coefficient of two mass transitions for each compound.
As a result of these studies, we identified putative slide acceptance criteria: array fluorescein mean element intensity >5000 RFU/pixel, coefficient of variation (CV) in intensity <10%, mean signal to noise score (signal/signal + noise; S/S+N) >0.85, and CV in spot size <20%.
Spots showing more than 2-fold changes in intensity with a coefficient of variation <50% and with statistically significant differences by Student's t-test (P < 0.05) were defined as differentially expressed.
To minimize the tailing effects, we computed the best fit using only the points within 25% from the maximum intensity, and computed the coefficient of determination R for this subset.
