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Normalised log2 intensities were deemed highly, well and lowly/not expressed when the intensities of total background distribution > 95th, at the 50th and < 20th percentile respectively.
Labelling intensities of total, nuclear and cytoplasmatic Cyclin D1 were obtained in each image using the integrated density option of the Image-J software.
Inside each cell, one gate for the nucleus, using DAPI as a marker, and one gate for the cytosol was drawn and threshold intensities of total pixels were determined for Numb.
The statistical analysis of peak intensities of total cell lysates of fibroblast NBCCS patients and control individuals revealed an elevated number of differentially expressed protein masses (56 in total: 35 in the range 1.5 30 kDa and 21 in the range 30 150 kDa (see Table 1 provided as Supplementary Material available online at http://dx.doi.org/10.1155/2013/794028).org/10.1155/2013/794028
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The fluorescent intensity of the resultant solution was measured and presented as F t, i.e., the fluorescent intensity of total curcumin.
b Doubling time values for longitudinal increase in bioluminescent intensity of total metastatic burden for individual control (blue) and treated (red) mice.
Mean DNA microarray signal intensity of total detected genes was significantly greater in organically managed soils than in conventionally managed soils (Table 7) [42].
Compared with MM alone, the average GFP fluorescence intensity of total bacterial cells (Total) of Ech3937 (phrpA) was doubled when 0.1 mM of OCA and TCA were added to the medium (Table 2).
It was then divided by the band intensity of total p38 for normalization.
Glycosylation efficiency was calculated as a percentage using intensity of glycoprotein over intensity of total (glycosylated and aglycosylated) protein.
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