Your English writing platform
Free sign upSuggestions(5)
Exact(6)
The detected signal exhibits a high or low phase shift, depending on the ratio of the intensities of the reference and the indicator.
The identified main sources of measurement uncertainty were uncertainty of calibration reference materials (C i ), uncertainty of delivered volumes, uncertainty of measured intensities of the reference solutions (A i ), and recovery of the method (Figure 1). Figure 1 Cause and effects diagram of uncertainties.
To calculate the cut-off per array, log2 values of the intensities of the reference MRSA252, Cy5 signal, on the MRSA252 and the non-MRSA252 spots were determined.
Fluorescence intensities of scanned images were quantified, normalised and ratios were calculated and compared to the intensities of the reference pool (Yang et al, 2002).
Box plots, in which the log2 values of the intensities of the reference MRSA252 (Cy5) signal for the MRSA252 and the non-MRSA252 spots are presented, indicate highly variable intensities between different spots on the same microarray slide.
In such cases the reference channel, which is normally designed to provide a large dynamic range of consistent signal intensities, will be the driving force of the ratio calculations and hence there will be a correlation between gene expression ratios and the signal intensities of the reference channel.
Similar(54)
(1) C N R (C N A T. t o t a l ) = log 2 S A + S B R A + R B (2) C N R (C N A T. a l l e l i c ) = log 2 S A R A + log 2 S B R B In the above equations, S refers to the intensity of the test sample (of an individual) and R to the (mean) intensity of the reference panel; A and B refer to the SNP alleles.
Due to the efficient donor acceptor energy transfer (96 97%) the acceptor fluorescence of the systems, excited in the maximal absorption of the donor chromophore, was enhanced more than 33 times with respect to the fluorescence intensity of the reference Rhodamine 6G.
At the same time, the emission intensity of the reference dye located in the core part of the particles kept almost constant under the different pH.
The PL peak intensity of the reference ZnMgO at 357 nm is normalized to one, and a sixfold enhancement in peak PL intensity is observed from the ZnMgO film capped with the Pt NPs.
For example, HelixTree and PennCNV-Affy establish a reference in this way, then use the log2 of the ratio of the hybridization intensity of an individual subject to the intensity of the reference (log2 R (subject/reference)) for further segmentation or for modeling copy number status [17].
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com