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Intensities of staining increased from well, moderately to poorly differentiated tumors.
A cell was considered as c-Fos-labeled (c-Fos positive), if the brown-black DAB-stained nucleus was unambiguously darker than background staining, which included all cells from low to high intensities of staining.
Average intensities of staining at cell rears include values in all bins posterior to the position of maximal DAPI fluorescence, while intensities for cell anteriors include values in all bins anterior to the position of maximal DAPI fluorescence.
The intensities of staining by ZNP-1 (control and Mn treated) and of tyrosine hydroxylase (control, Mn treated and recovered) in images were analyzed by choosing identical regions of interest (ROI).
Scoring for RANK was based on relative intensities of staining of tumor cells with reference to the normally present RANK staining of tissue associated macrophages.
Different intensities of staining were not analyzed separately.
Similar(47)
Immunostaining was assessed by semiquantitative scoring systems for percentage of cells staining and intensity of staining.
This reflected both an increased proportion of cells staining and increased intensity of staining.
The intensity of staining (scale 0 4) and the percentage of stained cells were evaluated.
Scores were given separately for the stained area and for the intensity of staining.
Three independent pathologists blindly reviewed each stained slide and semiquantitatively categorized the intensity of staining.
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