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Finally, the fluorescence intensities of each sample were used to compute the PBF PBFMSS) following the standard MS reference technique [23].
A table of time-aligned detected features containing the retention times, m/ z ratio, and intensities of each sample was then obtained.
The methylated and unmethylated band intensities of each sample were visualized and measured using Storm840 and ImageQuant Software (Amersham Biosciences, Little Chalfont, UK).
Since samples from different time points are from the same reaction, the distribution of fluorescence intensities of each sample should not change over time in the absence of aggregation.
To account for deviations in overall intensity due to volumetric errors in sample collection and preparation as well as systematic drift or random fluctuations in ionization efficiency and mass detector sensitivity, peak intensities of each sample were normalized to the internal standard (ribitol) peak.
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The diffraction intensity of each sample is presented logarithmically to better observe the background levels.
The fluorescence intensity of each sample was measured using a spectrofluorometer with excitation and emission wavelengths of 760 nm and 820 nm, respectively.
Simultaneously, it can also be found that the absorption intensity of each sample (TNTAs, PCTs, and BTs) is gradually increasing after their absorption peaks.
The fluorescence intensity of each sample well was measured by microplate reader (GENios, Tecan, Switzerland) with excitation wave length at 430 nm and emission wavelength at 485 nm.
The relative fluorescence intensity of each sample solution for each investigated drugs was accurately measured and plotted against the final drug concentration (ng mL-1) to get the calibration graphs.
The peak intensity variation among 32 samples was normalized by fixing the median intensity of each sample at 10,000.
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