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According to the fluorescence intensities, cells bound by BA-hBD3 and BA-Hst 5 were only 10 15% of that found for BA-LL37-treated cells (Fig. 4C).
Based on these intensities, cells were sorted into four fractions (orange, blue, purple, red) by FACS.
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The mean intensity of the high-staining subpopulation was about twice the mean of the low-intensity cells.
semiquantitative analysis showed the percentage of Nav1.8 immunofluorescent positive (>20% maximum intensity) cells in small, medium and large DRG neurons.
βcat fluorescent intensity: Cells were outlined, mean intensity measured, background subtracted, and βcat average intensity of a transfected cell normalized to mean of the βcat intensity of 2 3 adjacent untransfected cells.
To measure total cell fluorescence intensity, cells were fixed with paraformaldehyde (3.7%, RT, 15 min) and permeabilized with 0.1% Triton X-100.
To assess quenching efficacy and total surface intensity, cells were labelled on ice for 30 min and either quenched or left unquenched.
Fluorescence microscopy allows for good contrast between the bright, but of possibly heterogeneous intensity, cell and the dark background.
The output report from Ariol® provides image information and data such as signal intensity, cell location in 'England Finder' (EF) co-ordinates and selected case information).
Data were expressed as relative fluorescence intensity (RFI=mean fluorescence intensity of cells stained with anti-FAP-1 Ab/mean fluorescence intensity of cells stained with control Ab).
(C ) Average G1 length binned by Whi5 tot intensity of cells with Cln3 intensity less than 300.
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