However, even if the above assumptions are correct, the comparable V0 among different stimulation intensities cannot be fully explained, because the estimated activity of 25% is still far from the maximal activation attained with supramaximal stimulation.
Signal intensities cannot be less than 0, so the initial step in the analysis using GeneSpring version 7 software required that all values less than 0 be converted to a positive value, (i.e., 0.01) as done routinely [ 16, 17].
It is not feasible to relate the intensity of immunoreactivity to channel activation; thus, the present results about differential TRPM7 and TRPM2 signal intensities cannot be extrapolated to a specific role of these proteins in neuronal survival or death.
It should be appreciated that while the signal intensity from each linkage-specific antibody can be compared among samples on a given blot, signal intensities cannot be compared between blots probed with the different antibodies as each antibody has a distinct affinity for its cognate antigen.
The observed inter-taxa differences in hybridization intensities cannot be attributed to differences in the amounts of DNA loaded on the gels as because ethidium bromide staining of the gels revealed little differences in DNA quantities/labeling intensities between lanes (data not shown).
