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Cover with saran wrap and pop it in film cassette with intensifying screen.
Autoradiography was performed with an intensifying screen at −80°C.
After electrophoresis the gels were fixed in 40% methanol containing 3% acetic acid for 10 min, dried and exposed to intensifying screen.
Autoradiography of the membranes was performed for 6 24 h at −70°C using Kodak BioMax MS film with a BioMax intensifying screen (Eastman Kodak, Rochester, NY).
Membranes were washed once at room temperature in 1× SSC+0.1% SDS, then three times at 68°C in 0.5× SSC+0.1% SDS, followed by overnight exposure to film with an intensifying screen at −80°C or a phosphor screen.
After incubation, slides were rinsed twice in cold 50 mM Tris-HCl buffer, pH 7.4, and once in deionized water, dried and exposed to Kodak BioMax light film with an intensifying screen for 3 weeks.
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(12)Intensifying screens.
The facility shall use intensifying screens for mammography that have been designated by the screen manufacturer as appropriate for mammography and shall use film that is matched to the screen's spectral output as specified by the manufacturer.
The major uses of gadolinium compounds include hosts for phosphors for fluorescent lamps, X-ray intensifying screens, and scintillators for X-ray tomography, and as a magnetic resonance imaging (MRI) contrast agent (in the form of water-soluble chelates).
Other dose-reducing techniques include long source-film distances (88%), rectangular beam limitation (47%), leaded aprons (95% for extraoral films; 85% with thyroid collars for intraoral films), and rare earth intensifying screens (100%).
Very efficient new red phosphors based on YNbO4 and doped by Eu3+, Ga3+, Al3+ allowed recommending these materials as good candidates for different applications including LED and X-ray intensifying screens.
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