The cells with apoptotic morphologies or cells with bright nuclei (intense, complete coverage of the nuclei with foci staining) were excluded from the analyses.
We considered HER2-positive those cases with 2+ (weak complete membrane staining in >10% of cancer cells) or 3+ grading (intense complete membrane staining in >10% of cancer cells).
Score + 2 indicates complete membrane staining in nonuniform or weak but with obvious circumferential distribution in = 10% of cells, or intense complete membrane staining in = 30% of tumor cells.
Based on the immunointensity of the tumor cell membranes, four categories were defined: 0, no staining; 1, weakly or patchily positive; 2, moderate; and 3, intense complete membrane staining (Fig. 1A D).
Her2 was scored from 0 to 3; 0 = no staining; a score of 1 = faint, partial staining of the membrane; a score of 2 = weak complete staining of the membrane in > 10% of cancer cells; a score of 3 = intense complete staining of the membrane in > 10% of cancer cells.
"The mirror came off and came through the side and hit me in the face and for a moment it was pretty intense," he completed.
Working with intense focus, he completed the scores in September 1828, about six weeks before he died in delirium at 31.
This is how, over the span of four intense months, Gilbert Fletcher completed the portraits of 20 great black writers, from Ralph Ellison to Maya Angelou to August Wilson.
Only nuclear reactivity was taken into account for ER, PgR and Ki-67, irrespective of the staining intensity, whereas only intense and complete membrane staining in >10% of the tumor cells were considered HER2 overexpression (3+).
As the armed Predators were being completed, an intense discussion emerged over their use.
