Desalting the protein of DMSO was important because the later manifests an intense natural abundance C peak from methyl groups at approximately 2.6, 41.5 ppm (H, C), around the same region where we expected to see Cβ carbon from cysteines involved in the disulfide bond in 2D H C HSQC.
Samples for HSQC experiments were dialyzed against 50 mM NaH2PO4, 50 mM NaCl pH 7.0 instead of 50 mM Tris-HCl, 50 mM KCl, pH 7.0, to avoid the intense natural abundance peaks from Tris in the 2D H-C HSQC experiments.
The availability of agricultural crops, intense solar radiation, abundance of water, diversity of climate, and being a pioneer in biofuel production on a large scale are factors that create great opportunities for Brazil in this context [17].
To date, after more than 40 years of intense research, an abundance of beneficial effects have been attributed to n3-PUFA.
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Abundances of the three most intense peptides were averaged to get a protein abundance value.
The contralateral femurs showed considerably less evidence of Cx43 staining (lower abundance and intensity) and tended to be present in discrete packets rather than the intense, continuous staining observed in the injured bone (data not shown).
Peptides of each protein were ranked by their average intensity over all samples and the three most intense peptides were averaged to obtain an abundance value for every protein.
Middle and lower shoreface sands are characterized by the relative abundance of argillaceous sediments and intense bioturbations.
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