An overview of all promoters used in the transcriptome and translatome data together with their intended tissue specificity can be found in Supplementary Data Table S1.
Targeting unique mRNA molecules using antisense approaches, based on sequence specificity of double-stranded nucleic acid interactions should, in theory, allow for design of drugs with high specificity for intended targets.
This concept is validated by dynamically examining structural adaptations promoted by imatinib redesigns intended to control drug specificity.
If specificity is intended by such a magic bullet, receptor ligands such as those targeting the dopamine and somatostatin receptors and most recently those ligands targeting the misfolded amyloid protein, are good examples of the progress achieved.
(This choice is intended to improve the specificity of the alert system for any given threshold).
In the present study, we devised and successfully implemented strategies for "technical" ploidy reduction, in which three categories of haploSNP sites were identified and targeted with probes intended to have subgenomic specificity.
The method validation intends to investigate specificity, sensitivity, linearity, precision, accuracy, recovery, matrix effect and stability according to USFDA guideline.
Here we employ facile recombinant antibody technology to subtractively select ligands to each of the 7 BoNT serotypes, resulting in populations with very high specificity for their intended serotype.
On the other hand, we showed also problems with the specificity of assays intended for confirmation in resource limited settings where implementation of confirmatory assays like Western Blot or Innolia was very early proven to be challenging because of the required logistics and the high cost [22].
Initial data demonstrated a specificity of the intended capture intervals of 35% with exon hit rate of 99% using 100-200 bp genomic fragments and protocols similar to the published results and Agilent custom 244 K oligo arrays.
We had intended to test the sensitivity and specificity of one POC against Determine™ but the test stocked by the clinic changed throughout the study period, depending on clinic purchasing patterns which we could not influence.
