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"I honestly believe if more people understood that there is a strategy and intended sequence of events with an end in sight, they would be tolerant," Mr. O'Hanlon said.
Therefore, codelivering the site-specific nuclease with an alternative repair template, such as a plasmid or single-stranded oligodeoxynucleotide (ssODN) bearing locus-specific homology and any intended sequence changes, enables precise mutations at or near the induced DSB.
This data shows that proteolysis of the parent peptides constituting each gel occurs at the intended sequence location.
ChIP-Seq not only revealed enriched binding of ZFPs to their intended sequence, but also considerable off-target binding, especially at promoter regions.
Consistent with the view that consciousness and action may be closely related, brain imaging studies have shown that delusions of control, often seen in schizophrenic patients, are associated with a failure in the mechanism by which the predicted consequences of an action are linked to the intended sequence of motor commands (Frith et alia 2000).
The dating of the sets is unknown, as is Blake's intended sequence for the illustrations.
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Plasmids were sequenced to confirm that they had the intended sequences.
All vectors produced for this study were sequenced to confirm that they had the intended sequences.
Higher depth of sequencing can improve coverage across difficult targets and as expected, the proportion of the genomic regions with high read depth was improved when intended sequencing depth was also higher with optimal coverage (30X) reached for 82.0% of the targets that were sequenced using the Agilent V3 kit that had an observed average depth of 80X.
After sequencing the vectors to confirm that they had the intended sequences, and confirming protein expression in HEK293 by transient transfection, an I-CeuI- and PI-SceI-treated fragment was subcloned into Adeno-X viral DNA (pAdeno-X-cMyc-FCoR) (Clontech).
Despite the use of different capture kits and different intended sequencing depths, the consistency in capture across exomes was maintained with very minimal inter-sample variability (over 85% of bases fell within ±5 reads, with the exception of the V5 capture kit data that only included 6 exomes and therefore 85% of bases fell within ±10 reads).
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