Sentence examples for intended mutations from inspiring English sources

All constructs were sequenced to verify the intended mutations.

We recovered numerous otherwise isogenic clones carrying the intended mutations and analyzed the effect of each on BAX pre-mRNA splicing.

This is likely the result of the ∼110 bp between the site of the ZFN-induced break and the site of the intended mutations.

All clones were sequenced to confirm that only the intended mutations were incorporated into the constructs.

All mutations were generated with the QuickChange mutagenesis strategy (Stratagene, La Jolla, CA) and were sequenced to verify the intended mutations.

Collectively, Southern and Western blot analysis indicated that the intended mutations were successfully introduced into the HSV-2 ICP0 gene.

We verified that the intended mutation led to a complete loss of coronin-1A in homozygous animals (henceforth referred to as Coro-1A−/−; Figure S1A B).

All mutants were sequenced to verify the intended mutation.

Six clones from each intended mutation were purified by restreaking.

This plasmid was designated pLAY581 and was sequenced (Laragen, Los Angeles, CA, USA) to verify the presence of the intended mutation.

Afterwards, these fragments were purified, mixed and used as template in a third PCR in order to obtain a 2,645 bp fragment bearing the intended mutation as well as flanking regions 3' and 5' by using the nested primers gna1aa5NF and gna1aa3NR, which in addition contains an artificial ApaI and a SalI restriction site to facilitate cloning.