All mutants were sequenced to verify the intended mutation.
Indeed, H3K4A and H4K16G mutants intended to mimic the charge of constitutively acetylated residues, as might be expected upon sir2 deletion or catalytic mutation, were found to have little impact on either maintenance or establishment of centromeric silencing.
To confirm that this interaction was conserved across species, we used an in vitro co-precipitation assay with human homologues to follow Hsp90 Sgt1 interactions in solution (see Materials and methods), and compared the interaction of wild-type Sgt1 with mutants intended to disrupt the conserved Sgt1 Hsp90 interface.
It was intended to delete the mutant SOD1 gene from the Schwann cells, thereby diminishing the expression of the mutant SOD1 in this cell type.
As CaZF could partially suppress salt sensitivity of hog1cnb double mutant we intended to analyze ENA1 expression, which is regulated independently by both Calcineurin and Hog1p, in CaZF expressing cells (Figure 7C).
Nonetheless, when we crossed the Dido3ΔCT mutation onto a p53-deficient background, a genetic manipulation intended to render Dido3 mutants resistant to p53-dependent apoptosis and growth arrest, we were unable to rescue the lethal phenotype of Dido3ΔCT-RFP mice (Supplementary Table 1).
In Atoh1 mutants, the neurons that are intended for the retrotrapezoid nucleus are born, but they fail to migrate to the ventral brainstem.
The result, in "Peacekeeping," is a cynicism about Haitian politics that the author himself may not quite share, and may not have fully intended — a toughened, macho realism that is the mutant offspring of good knowledge and good technique.
She jumps in front of cure darts intended for Magneto and, after she loses her mutant abilities as a result, Magneto abandons her.
We intended to point out that in the vti11 mutant vacuolar morphology is still differentially regulated in tricho- and atrichoblast.
