Sentence examples for intended deletion from inspiring English sources

Again, all of the colonies that had lost the selection cassette after induction of I-SceI expression had the intended deletion.

The deletion cassette to delete 83 kb of the scaffold 15 was constructed in the pRS426 plasmid [ 54] and contained the hygromycin resistance cassette flanked by 1 kb fragments from both sides of the intended deletion.

Southern blot analysis of ten tetracycline resistant transformants demonstrated the intended deletion in the mg192 gene (Fig. 8C).

Each PCR product was sequenced with internal primers to determine if the intended deletion had been introduced.

That is, each read mapped to the intended deletion region was removed with probability of belonging to the fetus and also being inherited from the intended parent.

Following an analogous conjugation and genotyping protocol as described for S. lividans, seven out of seven exconjugants isolated with the phpD-targeting plasmid were confirmed to possess the intended deletion.

We confirmed the integrity of the intended chromosomal deletion mutations by DNA sequence analysis of the chromosomal locus from each strain using PCR primers listed in Table 3.

Nucleotide sequencing of the 1684 bp fragment confirmed the presence of the intended in-frame deletion.

Nucleotide sequencing of this 2078-bp fragment confirmed the presence of the intended in-frame deletion.

The intended gE gene deletion and insertion of the βgal gene at the gE locus was further confirmed by Southern blot analysis with a gE-specific probe.

The proposal, on which Icann is seeking feedback via e-mail messages sent to [email protected], is intended to prevent unintentional deletions of domain registrations.