Images were acquired with a CCD camera using Slidebook imaging software (Intelligent Imaging Innovations).
The imaging system operated under control of SlideBook 6 (Intelligent Imaging Innovations, Denver, CO).
Confocal time-lapse imaging during in vitro culture was performed using spinning-disc microscope system (Intelligent Imaging Innovations).
Extended Experimental Procedures Time-Lapse Imaging Confocal time-lapse imaging during in vitro culture was performed using spinning-disc microscope system (Intelligent Imaging Innovations).
Time-lapse imaging of labeled RNA was performed using a spinning-disk confocal microscope (Marianas; Intelligent Imaging Innovations) with Slidebook (Intelligent Imaging Innovations) software and a camera (QuantEM 512SC; Yokogawa).
SlideBook 5.0 software (3I intelligent Imaging Innovations) and sCMOS (Andor) Neo camera were used for the image acquirement and recording.
Subsequent imaging was performed with an IX81 microscope (Olympus, Center Valley, PA, USA) running Slide Book 5.0 software (Intelligent Imaging Innovations, Denver, CO, USA).
The microscope, camera, and data were controlled by SlideBook™ software (SlideBook™ (Intelligent Imaging Innovations, Denver, CO, USA).
The company is a spinout from Intelligent Imaging Systems, a Canadian road technology company that has been around for a decade.
Cell perimeter was measured for each cell in the population using Slidebook™ (Intelligent imaging, Inc).
It was also equipped with a motorized spherical aberration correction unit (Intelligent Imaging Innovations, Denver, CO).
