Exact(2)
The control was always included to normalize each reaction with respect to RNA integrity, sample loading and inter-PCR variations.
The transcript of actin gene was used to standardize each reaction run with respect to RNA integrity, sample loading and inter-PCR variations.
Similar(58)
As disintegration or lysis comprises cell integrity samples were inspected under a light microscope prior to freeze-drying.
The collection, storage, and associated sample logistics were coordinated to ensure sample integrity: samples were kept cold until being sent in cryogenic containers filled with dry ice.
These data were further authenticated for integrity and sample uniformity.
All probes demonstrated a comparable slope, indicating similar integrity across sample probes.
Serotype and sequence type were identified as described in [ 35] to check the integrity of sample handling.
The collection of additional samples requires storage methods that preserve the integrity of samples over the entire storage period.
To achieve model integrity, 90 samples comprising reference materials of cracked spores, uncracked spores and concentrate of Ganoderma lucidum were used as markers in our model building.
There are producers out there that are capable of generating the emotional integrity that sampling so easily provided.
Following verification of their integrity, RNA samples were reverse transcribed with Superscript II (Invitrogen), following manufacturer's instructions.
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