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The images also indicated that the nanoparticles induced increasing cell death in a concentration-dependent manner but with no disturbance of cell membrane integrity (Figures 3A and 4A).
The other main morphological differences between the two strains are spore shape, and the maintenance of spore chain integrity (Figures 3 4).
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In addition to these threats, an often neglected challenge facing in situ conservation is that of gene flow between cultivated and wild rice species and its impact on genetic diversity and integrity (Figure 4).
This structural transition is not due to a change in histone composition or integrity (Figure 3B).
Finally, karyotype analysis revealed that ASThiPS cell lines had normal chromosomal integrity (Figure 4C).
Thus, loss of γCOP or sar1 does not affect epithelial integrity (Figure 5E, J, O).
Occludin concentration was analyzed before and after stretch as a marker of TJ integrity (Figure 5).
The nuclei were stained with PI, indicating the loss of the plasma membrane integrity (Figure 3L, P).
Similarly to the ΔSPI-2 strain, infection with any of these three mutants failed to impact on PB integrity (Figure 3D and Figure S3).
The assembled scaffolds were analyzed by scanning electron microscopy (SEM) and evaluated in terms of ceramic-polymer fitting, DBM infusion, and overall construct integrity (figure 2).
However, this was ruled out by the observation that incubation of HeLa cells with purified Salmonella LPS did not affect PB integrity (Figure 3A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com