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The impact of PTEN downregulation was analyzed on cell polarization and differentiation, intercellular junction integrity (expression of cell-cell adhesion proteins, barrier function), migration (wound assay), invasion (matrigel-coated transwells) and on tumor and metastasis formation in mice.
In order to gain further insight into the manner by which PTEN controls junction integrity, expression levels of several key junctional proteins such as occludin, ZO-1 and claudins [2] were analyzed.
We created a variable reflecting the integrity of the E-cadherin/β-catenin complex in the cellular membrane, which included two categories: retained integrity (both molecules showing a membranous pattern) and lost integrity (expression of at least one of them not observed in the membrane).
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And since it is here that someone wrestled with ideas, sought integrity of expression and gave expression to fresh-jacketed voices, the book-studded room seems sacred.
Like Mr. LaChiusa, Mr. Bucchino possesses a singular voice and an absolute integrity of expression.
But there's a caveat at the end of the document exempting all signatories from the rules listed earlier ("our aim is integrity of expression, above and beyond any commitment to form") and a hilariously sulky tone throughout.
Nevertheless, those SVs may affect gene integrity and expression.
The histone methylation balance on specific residues in particular is crucial for maintaining genome integrity, gene expression and evasion of cancer [ 10, 52, 53].
To assess the impact of loss of AP1 factor functional inactivation on barrier integrity, TAM67 expression was induced on E14 and embryos were collected on the day before birth (E20) to assess barrier integrity.
To determine survival, oxidative stress, phosphatidylserine externalization and loss of membrane integrity upon expression of αSyn, cells from overnight cultures were inoculated in SCD to OD600 0.1, grown to midlog phase and shifted to SCG for induction of αSyn expression.
As the full reading frame and regulatory elements of th were maintained by using this knockin strategy, both the integrity and expression pattern of the gene product should be normal.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com