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The absence of variation in the third NMR signal component with leaf ageing that is generally attributed to plastids (Musse et al. 2013) and observed for both genotypes and conditions was in accordance with the preservation of plastid integrity estimated by micrographs and confirmed by physiological parameters.
To assure that the decreased number of VSMC upon treatment with capsaicin is not due to cytotoxicity, we quantified cell death by measuring cell membrane integrity estimated by lactate dehydrogenase (LDH) activity inside cells and in cell supernatants.
DTI allows the measurement of fiber integrity through estimates of fractional anisotropy (FA) and mean diffusivity (MD), which presumably relate to changes in axonal diameter, density, and myelination (Jones 2010; Jbabdi and Johansen-Berg 2011).
IMR evaluations of the coronary circulation seek to quantify microvascular integrity by estimating minimum achievable microvascular resistance, a related measure to maximal vascular flow reserve assessment with CFR.
The RNA integrity was estimated using denaturing electrophoresis on 1% (w/v) agarose gels.
cRNA amounts and integrity were estimated by intensity of fluorescence in ethidium bromide-stained agarose gels.
The concentration and integrity were estimated based on the control band intensity in the CHEF analysis.
RNA integrity was estimated by resolving 1 μg of sample in a 1%% (m/v) ethidium bromide agarose gel.
The integrity was estimated using a RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent technologies, Santa Clara, CA).
RNA integrity was estimated via capillary electrophoresis in an Agilent 2100 Bioanalyzer (Agilent Technologies, Naerum, Denmark) using RNA 6000 Nano Kits (Agilent Technologies).
The quantity and purity of the extracted total RNA was measured by spectrophotometry while the quality and integrity was estimated by gel electrophoresis.
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