The overlapping genes are mostly associated with cell division, cell growth, kinase activity, translation, DNA integrity checkpoint and transcription regulation.
The third functional group was response to UV damage, and included the GO categories "response to UV", "DNA damage checkpoint", "DNA integrity checkpoint", and "double-strand break repair".
Under the conditions we used in this paper activation of p38 kinase was not observed after siRNA treatment, arguing against activation of the proposed centrosome integrity checkpoint.
The other four down-regulated gene sets were related with cell cycle and cell division, such as DNA integrity checkpoint and mitosis.
In this subset of cited genes, a significant enrichment of genes associated with cell cycle regulation, protein kinases, DNA integrity checkpoint and protein metabolism was observed.
Consequently, these results suggested that it would be difficult to decipher whether a centrosomal protein is directly involved in ciliogenesis or not, as its depletion could trigger the centrosome integrity checkpoint.
Depletion of centrosomal proteins has been proposed to trigger a centrosome integrity checkpoint, resulting in p38 kinase-dependent cell cycle arrest (Mikule et al., 2007; Srsen et al., 2006).
Downstream of ATR are Chk1 and Chk2, in which Chk1 functions in the activation of the homologous recombination repair machinery [ 33] and, in metazoans, in the activation of the origin firing and replisome integrity checkpoint [ 34, 35].
The main phases regulated by CDKs are the DNA integrity control checkpoints, mediated by the retinoblastoma susceptibility gene suppressor (Rb), the tumor suppressor gene TP53, and transcription factors of the E2F family [ 4, 5].
INO80 and SWR1 probably function antagonistically in regulating genome integrity and checkpoint adaptation, which is the escape from an extended cell cycle checkpoint [ 112, 114].
