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The coding sequences for the 3H6 Fab heavy and light chain were both integrated on a pYX integrative expression vector under control of the constitutive S. cerevisiae TPI1 promoter.
yeast integrative expression cassette.
yeast rDNA integrative expression cassette.
The expression plasmids contained a YRC sequence (yeast rDNA integrative expression cassette) for homologous recombination or a YIC sequence (yeast integrative expression cassettes) for non-homologous recombination.
As a platform of recombinant protein expression K. lactis furnishes several advantages including applications of both episomal and integrative expression vectors, simple fermentation tools and easy genetic manipulation.
For this purpose, we constructed an integrative expression vector (pEPG-FLO5; Figure 1B) containing the FLO5 gene from S. cerevisiae under the control of EPG1, a promoter not repressed by glucose and induced by anoxic conditions.
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Based on Sawatzky and Pesut's [ 13] definition (presented in the introduction), integrative expressions of spiritual care rest on nurses' awareness of the transcendent dimension.
We carried out a full-scale integrative co-expression analysis of gene expression in a large panel of rat recombinant inbred (RI) strains, the BXH/HXB panel [27].
Oncomine databases for integrative gene expression and copy number variations (CNVs) analyses in liver tissues.
For an integrative gene expression analysis, we combined three microarray datasets into a single dataset by adjusting batch-specific effects using ComBat method [ 15].
The integrative gene expression cassettes were designed to minimize the number of transformations and the remaining loxP scars after removal of the selection markers.
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