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Mapping of T-DNA flanking sequences demonstrates 67%% of T-DNA integrations are integrations at a single chromosomal site and 31%% of T-DNA integrations are associated with large-scale chromosomal rearrangements.
A high density of A. tumefaciens increased the transformation frequency; however, undesirable integrations of the T-DNA such as multiple-copy integrations at a single locus, with or without the vector backbone, or integration at multiple loci also increased in A. aculeatus, as previously reported in Blastomyces dermatitidis (Sullivan et al. 2002) and in Suillus bovinus (Hanif et al. 2002).
However, due to the potential for PB transposon local hopping (Wang et al., 2008), multiple integrations at a given genetic locus within a single screen were considered as one hit.
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Mice lacking the p27Kip1 Cdk inhibitor (Cdkn1b) exhibit increased susceptibility to lymphomas from the Maloney murine leukemia virus (M-MuLV), and exhibit a high frequency of viral integrations at Xpcl1 (Kis2), a locus on the X-chromosome.
Their curricular model is more integrated than ours, which allows for better longitudinal coordination and integration at a higher level.
Sequence-specific integration at a preferred pseudo attP site in the mouse genome was detected by using PCR.
The miniaturization of such a beam-deflection system facilitates its integration at a favorable place within the electron-optical column.
By his life and work, he brought the Persian and the Islamic cultures toward a closer integration at a time when medieval Islam reached its zenith.
The South American hinterland is currently undergoing unprecedented spatial transformations, particularly through fast-paced modes of resource extraction and regional integration at a continental scale.
These words were uttered by the Italian politician Roberto Calderoli in reference to Cécile Kyenge, Italy's black minister of integration, at a recent festival organised by his party, the Northern League.
We also describe an additional mutant, P3 that combines the mutations of P2 with further changes and possesses an elevated specificity for integration at a chromosomally placed attP site in human cells.
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