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ACH3 is the integration value of the methoxy protons of the ME (the strong singlet) and ACH2 the integration value of the methylene protons.
Global integration value of city ranges from 0.44 to 1.24, Local integration range from 0.33 to 2.96.
We computed the integration value of each cell by using the analysis tools in space syntax and visualized the values with colors.
Based on the equation C = 100 ( frac{2A1}{3A2} ) [C is the percentage of triglycerides to corresponding methyl esters, A1 stands for the integration value of protons of the methyl esters (1.000) and A2 is integration value of α-methylene protons (0.723)] [25, 26], the methyl esters yield of 92.21% was calculated for the mentioned sample.
The equation used to quantify the conversion was [13]: {text{C}} = frac{{2A_{Me} }}{{3A_{{{text{CH}}_{2} }} }}(100% ) (1 where: C = percentage conversion of triglycerides to the corresponding methyl esters; AMe = integration value of the methoxy protons of the methyl esters; ({text{A}}_{{{text{CH}}_{2} }}) = integration value of α-methylene protons.
The integration value of luminescence intensity of each spot was measured with an ATTO Light-Capture (ATTO Corporation, Tokyo, Japan) which has a resolution of up to 62.5 µm, and analyzed by using CS Analyzer ver2.0.
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To reach more insights into the polymer structures, the unit ratio of 3-hexylthiophene (3HT) versus N-benzoyl dithieno[3,2-b 2′,3′-d]pyrrole dithieno[3,2-b 2′,3′-d]pyrrole dithieno[3,2-b 2′,3′-d]pyrroleiophene-CH2 proton signal at 2.6 ppm (peak f, Fig. 2a) and the BDnzoyl ortho proton signal of N-benzoyl dithieno[3,2-b:2′,3′-d]pyrrole at 7.7 ppm (peak o, Fig. 3a).
Retention times and integration values of peaks were compared to the chromatographic profile obtained for a standard mixture of derivatized amino acids.
The Mann-Whitney test was used to compare the peak integration values of the three group's breath tested.
The phosphate to TEAH+ ratio was estimated by comparing integration values of H NMR signals of ribose (or alkyl/aryl for 10 and 11) and TEAH+ protons.
The peak integration values of 2-AA can be regarded as semi-quantitative only, but are consistent with the results of the analysis for presence or absence of 2-AA in breath, and support the conclusion that the likely source of the 2-AA are Ps.
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