Exact(27)
Potential inhibitors would bind to two metal ions in the active site of HIV IN to prevent human DNA from undergoing the integration reaction.
The reporter genes were efficiently introduced into the CHO genome in a Cre-dependent manner, and transgene expression was detected after the integration reaction.
The analysis of the latter reaction catalyzed by the R/TD recombinase pair shows that the condition supporting the most efficient replacement reaction favors efficient TD-mediated integration reaction while favoring efficient R-mediated integration and deletion reactions.
The integration reaction was estimated essentially as described previously [22].
The role of BER proteins during lentiviral infection appears to be associated with the integration reaction.
We modified the in vitro integration reaction protocol and developed a reaction system with higher efficiency.
Similar(33)
All the integrases that were active on integrated DNA also promoted DNA integration reactions that were not mediated through conservative site-specific recombination or damaged the recombination sites but the extent of these aberrant reactions varied over at least an order of magnitude.
The gene integration reactions were repeated four times in vitro using Cre protein and specific plasmids.
The expected integration reactions mediated by Cre occurred at the loxP sites, resulting in integration of four target genes.
While autism can certainly contain environmental exacerbators (think of sensory integration reactions to certain sounds, sights, or tastes--experiences that bring out more behavioral differences through discomfort.
Standard concerted integration reactions were performed as described previously [16], [17].
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