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The deep sense of community and always-connected lifestyle that has resulted in their work-life integration preference is why millennials measure productivity by work completed, not by time spent on a task -- especially not time spent in the office.
Besides, T-DNA exhibits certain integration preference and may therefore not saturate the entire rice genome [8].
Whereas no ISAba1 copy was identified on any of the plasmids of isolate AYE, chromosomal copies showed no site integration preference (Figure 3).
Based on the TSS-proximal/intragenic/intergenic annotation of each integration site, we grouped datasets that do not significantly differ from each other (two-sided test on equal proportion) into seven groups of integration preferences, and generated the same number of random weighted control groups of sequences that reproduce, in proportion, the specific integration preference of each vector.
Understanding the integration preference sites for each respective class may shed light on the order of integration events.
We also validated plasmid constitutive expression, reporter function and integration preference, which did not alter host cell density or AP-3 levels.
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Bound transcription factors may very well be the cellular mediators of the LTR-associated component of RV integration preferences.
On the contrary, similar LTR modifications (ΔU3-HIV[CMV], ΔU3-HIV[MLV] and MLV-HIV) had no apparent consequence on the LV integration preferences (Figure 1).
Different retroviruses show significantly different integration preferences [2] [4], implying that PICs recognize components or features of the host cell chromatin in a specific fashion [5] [7].
It has recently been suggested that HIV-derived LV vectors, due to their different integration preferences and LTR enhancer-free design, could be associated to a lower genotoxic risk compared to conventional RV vectors [14], [25], [38], [39].
Based on the annotation reported in Figure 1, we generated seven weighted control groups of random sequences that reproduce, in proportion, the integration preferences of each vector set (Table S1).
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