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Cufflinks [ 13] assembled the results of TopHat into transcript assemblies, followed by the integration of transcript assemblies from different tissues.
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Integration of transcripts and metabolite accumulations was used to reveal the relation between IiPALs and target compounds.
The strength in our experiment however is the integration of transcripts, proteins and metabolites, to obtain significant biological information.
We have demonstrated that the integration of copy number and transcript profiling data provides effective risk stratification of men with localised prostate cancer in two novel, distinct cohorts totalling 259 men.
Intronic HERV-W provirus integrations on the sense strand appear to have elicited a weaker negative selection than pseudogene integrations of transcripts from such elements.
Therefore, the transcription profiles presented here represent the integration of the transcript levels of different homeologs of each gene.
A systematic integration of copy number changes with transcript array data and DNA sequence changes is currently underway in this and a larger set of sarcomas, which will provide a broader genetic landscape of adult soft-tissue sarcoma [Barretina J, Taylor BS et al. 2008, in preparation].
A straightforward extension of this strategy also allows for integration of partial transcript information.
Integration of the transcript, radiotracer and metabolite data demonstrates that Asc and TA metabolism are developmentally regulated in grapevines; resulting in low accumulated levels of the biosynthetic intermediate Asc, and high accumulated levels of the metabolic end-product TA.
Building such networks in other plant species will enhance inter-species comparison and deeper analyses through integration of already available transcript, protein and metabolomics data.
The integration of LOH with transcript expression data revealed 15 genes that displayed decreased expression and mapped to regions of LOH.
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