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Figure 5 Representative sections showing different grades of integration of repair tissue to parent cartilage.
It involved 8 categories: (A) cell morphology, (B) matrix staining, (C) surface regularity, (D) thickness of the defect, (E) integration of repair tissue with the surrounding articular cartilage, (F) arrangement of repair cartilage, (G) remodeling of subchondral bone, and (H) effects on adjacent cartilage.
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Integration of repaired cartilage with surrounding native cartilage is a major challenge for successful tissue-engineering strategies of cartilage repair.
After ablation of scars, the OECs from LP grafts increased the size of the cellular and axonal zones, more importantly, the absence of scar formation, the integration of repaired tissue with spared tissue, and remyelinated axons in the axonal zone were observed [ 94].
Three chitosan implant formulations reproducibly elicited neutrophils which guided osteoclasts to the bone plate, delayed deposition of collagen and glycosaminoglycan, induced subchondral bone resorption and repair and improved integration of the repair tissue with the subchondral bone.
The ICRS grading system involves 3 criteria: the degree to which a defect is filled by repair tissue, degree of integration of the repair tissue with the adjacent articular cartilage, and macroscopic appearance of the surface of the repair site.
The integration boundary of repair tissue with the adjacent host cartilage was morphologically and biomechanically evaluated at 6 months post-implantation.
Osteoclast-induced bone remodeling in the bone plate area of treated defects was associated with a 10-fold greater lateral integration of treated repair tissues with the bone plate compared to control defects with detached repair (p<0.05, Figures 11 and 12).
Good integration of the repair tissue to the parent cartilage was seen in the scaffold only and rhFGF18 groups, however in the BMP-7 group there was poor lateral integration (Figure 5).
At 6 weeks histological sections representative of the treatment groups showed areas of chondrogenesis with increased proteoglycan deposition and integration of the repair tissue with the native cartilage matrix.
Integration of the repair oligonucleotide into the chromosomal wild-type cdt-1 gene or cdt-1 Q45H/F262Y/F533Y was performed as described for integrating barcoded DNA.
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