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The integration of constructs post-implantation relies on the rapid formation of functional vasculature.
A major challenge for cartilage tissue engineering remains the proper integration of constructs with surrounding tissues in the joint.
Integration of constructs conveys carboxin resistance.
We show that the sdi1 locus is a useful site for targeted integration of constructs.
We establish the sdi1 of Z. tritici locus for targeted integration of constructs as single copies.
Correct integration of constructs P1 and P2 were confirmed by Southern analyses of separated chromosomes and diagnostic PCR analyses (Fig. 3B).
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The system is an international lighthouse project showing the potential for aesthetical integration of constructed wetland systems in the built environment.
Genomic DNA of blood stages of WT or mutant parasites was isolated as described[36] and analyzed by LR-PCR using primer pair (p1, p2) 3258 (5'-TAAACCTATTTGAAGCTTTATAC) and 3259 (5'-CTTGTGGGAAATTACAATGAC) for correct integration of construct p5236FRT in the pf52/36 locus by double cross over integration.
These have involved both multiple integrations of one construct into different locations [ 75] or integration of multiple constructs into one site on the X chromosome [ 13].
Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components.
Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.
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