DNA biosensors (or genosensors) are analytical devices that result from the integration of a sequence-specific probe and a signal transducer.
This method allows for the integration of any sequence of interest in a pre-defined locus, leaving Cre recombinase available for downstream applications.
Integration of a SINE sequence at a specific site in the genome is irreversible, and its target site is chosen almost at random [ 52].
The method involves the introduction of a novel cut site into a specific genomic location, followed by the integration of an edited sequence into the same location in a scarless manner.
During preparation of this manuscript, Yang et al. reported that stable integration of an antisense sequence against miR-210 (iR-210) suppressed cell viability, induced cell arrest in the G(0)/G(1) phase, increased apoptotic rate and enhanced radiosensitivity in hypoxic human hepatoma.
The approach encompasses three phases: technology assimilation, technology insertion and technology integration, following a sequence of modular, top-down and bottom-up design strategies.
To investigate the possibility of an integration of an REV gene sequence into the FWPV genome, we designed another 2 sets of primers for the amplification of a partial REV env gene and the REV env FWPV open reading frame 203, which contains the entire REV 3′ long terminal repeat.
M5NR [ 19], MG-RAST uses to annotate sequences, is an integration of many sequence databases into one single comprehensive, searchable database including most common functional databases like KO, EBI, and SEED.
Analysis of evidence from participant reflections and conversations during and after the institute revealed a teacher growth in envisioning MEAs as a vehicle for STEM integration informed by a sequence of professional development design decisions.
The basis of generation of this model is a strategic integration of extensive sequence, structural and evolutionary analyses with molecular dynamics simulations.
