Sentence examples for integration machinery from inspiring English sources
Of these integrating hcAd-based vector systems, those that capitalize on the DNA integration machinery of AAV have the advantage of allowing preferential insertion of foreign DNA into the so-called AAVS1 locus on human chromosome 19 (19q13.3-qter).
These transfers have been termed NIRVs (non-retroviral integrated RNA viruses) because the integrated elements differ from endogenous viruses in their requirement for co-option of integration machinery and in their normally subgenic architecture [ 3, 4, 10, 11].
Our integration machinery is broken.
A significant bias of lentiviral integrations in genes is reported, confirming that biological properties of the viral integration machinery facilitate preferred insertion into actively transcribed genomic regions.
Retroviral vectors are considered particularly useful vehicles for a GOI because the retroviral integration machinery is well suited to introduce a single intact copy into a subclass of apparently favorable genomic sites.
Among the many viral and non-viral vector systems capitalizing on the AAV integration machinery are those based on hcAd vectors [14], [38], [39].
It remains unknown, however, whether lentiviral integration can be directed by alternative integration machineries, despite the integrity of the PIC and the exquisite involvement of cellular factors in nuclear entry and chromatin association.
In addition, the 5' flanking sequences of rhesus monkey SNORA76b and SNORA24b possess T2A4 motifs, which are preferentially recognized by the L1 retroposon-encoded nicking endonuclease, suggesting that SNORA76b and SNORA24b were generated from a parental copy by L1 integration machinery-mediated retroposition.
Together, our findings provide a novel tool for Flp-based genetic engineering and pave the way for future applications of lentiviral DNA circles as potential substrates for more therapeutic relevant nonviral integration machineries in somatic gene transfer.
Its main products include low frequency converter, integration and machinery and servo systems.
The donor amplicon carries the mutant sequence and extensive flanking segments of homology, which ensure efficient and precise integration by the recombination machinery specific to competent cells.
