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Due to the fact that Flp-In system utilized Flp recombinase mediated transgene integration into single Frt site previously inserted in the genome of Flp-In™HEK293 cells, the clones generated should be isogenic and have single copy integration of STARS transgenes.
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Their integration into a single, integrated model properly described fire-proneness and is suggested to be a valuable tool for the identification and management of fire risk.
It requires developments in different engineering disciplines and their integration into a single system without degrading their individual performances.
Increasing the ratio of A. tumefaciens to target conidia to 1 × 104 improved the transformation frequency to 30 transformants per 1 × 104 conidia (Table 3, 48 h) on average; the single-copy integration of the T-DNA decreased to 30%, but the frequency of T-DNA integration into a single locus with the vector backbone typically increased to 55%.
But even as the integration into a single carrier was hitting its stride, Delta's operations struggled.
As a question of fairness, Europe's poorer eastern periphery needs better integration into the single market.
Designed in 1990 by David Hammons and titled "African American Flag," it unites ideals of separation and integration into a single image, much as the Studio Museum itself does.
One is that the hybrids manifest little or no reduction of fitness, so that gene exchange between the two populations proceeds freely, eventually leading to their integration into a single gene pool.
This economic recovery resulted partly from continuing integration into the single European market and from the government's stability plan, which reduced budget deficits and inflation and stabilized the currency.
Since these subsystems have different physical characteristics, their integration into a single system level unit makes the optimization problem of synthesis/design and operation/control highly complex.
Using the parts list of the reference structure, Fig. 3, components suitable for integration into a single cast part due to their arrangement, function and specific costs were identified.
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