Social integration was inserted into the models because of its importance for subjective well-being (as discussed in the "Social relations, friendship, and life satisfaction" section) and was measured considering the participation in meetings organized by political parties, trade union organizations, or by other (e.g., voluntary or cultural) associations in the year prior to the interview.
To ensure that all forms of Ds were expressed at the same level, we used site-specific integration to insert the transgenes at the same chromosomal location.
The resulting integration vector was inserted into the bacterial attachment (attB) site of E. coli using a helper plasmid, pAH121, expressing the phage integrase, by direct transformation, as described by Chiang et al. [ 26].
To ensure equivalent expression levels, both transgenes were inserted in the same genomic location using ΦC31-directed site-specific integration (insertion site 86Fb).
SB100X has a number of advantages over virally mediated gene delivery: it integrates transgenes exclusively into TA dinucleotides with specific predilection for dinucleotides that have distinct structural features, integrates transgenes with high efficiency, results in stable integration, integrates large inserts, and does not primarily integrate into transcriptional sequences [ 42– 42].
When multiple genes need to be integrated, a dispersed nature of integration sites causes the inserted DNA fragments to be more stably maintained than when tandemly inserted (Da Silva & Srikrishnan, 2012).
Because of the apparent widespread nature of transvection between transgenes inserted at integration sites, caution should be exercised when using these genetic tools.
Therefore, we proposed that Et-LEE may either have lost mobility after integration or have been inserted in a different manner than E. coli LEE.
An additional degree of difficulty is given by the fact that the existing plant nuclear genomes are the outcome of the balance between ongoing processes of integration and elimination of inserted plastid DNA by genome shuffling (Matsuo et al., 2005).
With the Atlassian integration, you can insert a project file directly in the email.
