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Furthermore, Southern blot analysis on monkeys #2 and #3 confirmed the correct transgene integration (Figure 1D) and the absence of no additional randomly integrated transgene (Figure 1D, internal probe).
The pyrG13 strain was transformed with the Aaku80 disruption cassette 2 and yielded transformants harboring the cassette integrated as a single copy, namely coku80, into the Aaku80 locus by homologous integration (Figure 4b, lane 2).
In most cases, participants actually achieved a good degree of integration (Figure 2(c)).
The participants continued to move for the entire two minutes of the experiment [Figure S5 in Additional file 1] but they eventually achieved the expected high levels of integration (Figure 2(b)) and high average scores (Figure 3(b)).
Good integration of the repair tissue to the parent cartilage was seen in the scaffold only and rhFGF18 groups, however in the BMP-7 group there was poor lateral integration (Figure 5).
Data on HIV integration site distributions in MEFs closely matched the data for EIAV integration (Figure 8 and Table 3).
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They could be products of classical transposase-mediated co-integration (Figure 4B a).
This duplicated molecule might then regenerate an eccDNA molecule by homologous recombination, allowing further integrations (Figure 8).
Since retroviruses can alter expression of genes up to 200 kb away [10], we analyzed expression of Slco5a1 in tumors with Evi32 proviral integrations (Figure 2a).
The different fragment sizes, visualized by Southern blotting, indicated that the transgenic construct was integrated into different locations of the genome in the tg60 and tg62 lines without any hint of multiple integrations (Figure 1C).
Open image in new window Figure 2 Integration design concept of 5-TET.
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