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As shown in Figure 4A, the number and intensity of hybridizing bands in ClaI digest reflected a complex integration event, with at least two integrated copies.
The GC content, which is close to 52%% for the three genomic regions and the integrated viral fragments, shows no traces of a recent integration event (Table 1).
With this integration, event organizers can now sell tickets in the app and on their promotional pages.
The fact that the size of this band (~10,000 bp) was much higher than expected (840 bp) strongly suggests the occurrence of an integration event.
Additionally, a fragment indicative of the correct integration event could also be detected (Fig. 3A).
Perhaps a viral genome became juxtaposed with a 7SK sequence during a particular integration event.
We analysed this parasite population by Southern blot, using a probe diagnostic for the specific integration event.
Southern analysis of tumors obtained from 27-186 and 18-077 indicated just a single retroviral integration event.
Among 45 embryos displaying at least one integration event of the transgene, 33 (73%) were LacZ positive.
Initially, we wished to verify loss of the tk gene from the clonal parasites, as this would be characteristic of a double crossover integration event.
In each sample there was at least 1 integration event per Mb on each chromosome, and in some samples the number was as high as 6.
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