Sentence examples for integration assays from inspiring English sources

This chapter describes in vitro integration assays, which are used to elucidate the enzyme mechanisms and to discover, design, and develop integrase inhibitors.

This also explains why, in our standard in vitro concerted integration assays, MAba4 strongly impaired the IN enzymatic activity.

The antibody incubated with IN inhibits its enzymatic activity in in vitro concerted integration assays [22], [48], [49].

Wild-type (WT) IN and IN with mutations Y143C or Y143R were assayed in vitro in 3'end-processing, strand transfer and concerted integration assays.

To learn more on the molecular mechanism underlying the integration inhibition, we also conducted concerted integration assays varying the preincubation conditions.

The stems reproduce several versions of the U5 LTR extremity of viral DNA The stem of LTR34 mimics the unprocessed 17 bp version of the LTR extremity, and is sufficient to carry out in vitro integration assays.

The injected oocytes were incubated under conditions specified for individuals experiments, then nucleic acids were extracted and electroporated into E. coli HMS174 DE3), followed by plating on LB containing ampicillin with or without tetracycline, as for the group II intron-integration assays (see above).

For plasmid-based group II intron-integration assays, target plasmid DNA (0.5 mg/ml) supplemented with 17 mM dATP, dCTP, dGTP, and dTTP (Invitrogen) and specified amounts of MgCl2 was injected into the posterior of the embryo, followed within 5 min by the injection of RNPs (1.6 mg/ml), using different needles to avoid prior mixing.

pBRR3-ltrB, the target plasmid for intron-integration assays, contains the Ll.LtrB homing site (ligated exon 1 and 2 of the ltrB gene from position −178 upstream to +91 downstream of the intron-insertion site) cloned upstream of a promoterless tetR gene in an AmpR pBR322-based vector [4], [8].

To test whether GDAP1 can integrate into membranes like a classical TA-protein, we established an integration assay similar to assays published by Henderson et al. [24] and Setoguchi et al. [17].

In order to develop an integration assay for patient samples, we enhanced the sensitivity of our prior integration assay.