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The SacI site of the pBS II SK backbone had been destroyed by site directed mutagenesis to facilitate the use of the endogenous SacI site in the 3'-DHFR-TS integrating sequence and the XhoI site as unique cloning site in pKOI.
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Integrating sequence and structural information yields an improved performance within the "twilight zone" of sequence similarity, which significantly extends the coverage of targeted gene products.
However, most alignment methods do not integrate sequence and structural information into one measure of similarity or describe the similarity at the level of individual residues.
In this paper, we present a probabilistic model called TRANSMODIS (TRANScription MOdule DIScovery) which integrates sequence and expression information in target identification.
In this paper, we introduce statistical depth function to define negative samples and propose an SVM-based method which integrates sequence and structural information to predict binding site.
In HepG2.2.15 cells, HBV DNA is carried as chromosomally integrated sequences and episomally as relaxed circular, covalently closed form of HBV genome [ 39].
In total, six raw assemblies were obtained for the six catfish and further merged by integrating sequence overlaps and eliminating redundancies for each species.
This comparison indicated they were of high quality, demonstrating the added value of integrating sequence- and expression information for gene function prediction [ 34].
In this study, an integrated algorithm is newly introduced to predict AMPs by integrating sequence alignment and support vector machine- (SVM-) LZ complexity pairwise algorithm.
Here, we describe a novel model-based approach RNAMotifModeler to identify the consensus of protein-RNA binding regions by integrating sequence features and RNA secondary structures.
Among these, 50 significant miRNA-mRNA interactions were predicted by integrating sequence target prediction, expression analysis, and the PageRank algorithm.
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